ZetaView® BASIC NTA – Nanoparticle Tracking Video Microscope PMX-120
For the analysis of size, concentration, fluorescence, electrophoretic mobility and sub-populations of individual nanoparticles
Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the ZetaView® from Particle Metrix.
Nanoparticle Tracking Analysis (NTA) captures the Brownian motion of each particle in a video. Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined. The concentration is calculated by dividing the number of particles detected by the fluid volume measured. , Finally, the charge state of the particle surface (zeta potential) is measured via the movement of the particles in an applied electric field.
Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles are also documented at each recording and can be used to distinguish sub-populations.
All these analyzes are carried out quickly and statistically reliably as required in the scattered light or fluorescence mode.
Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labeled with corresponding intercalating dyes.
Depending on the type of sample and the measuring mode, the measuring range is between 15 nm and 5 μm.
Your benefits at a glance
Automated measurements taken at 11 distinct positions in the sample cell offer a more representative analysis than single-point measurements. Outlier control selects flawless videos. Autoalignment and Autofocus keep the video quality constant.
No other nanoparticle analysis method offers more analysis parameters per sample. At high throughput, in scattering as well as in fluorescent mode.
NTA size and NTA zeta potential are absolute measurements
Time consuming and costly calibration procedures do not apply.
Z-NTA Surface charge mirrors surface status of nanoparticles
Quantity and type of proteins in the surface of e.g. extracellular vesicles particles or chemical nature of the surface influence the surface charge. Easy to measure in ZetaView® by means of the electrophoretic mobility.
The electrophoretic mobility v / E is determined by applying an electric field E and measuring the resulting velocity v of the particles. For practical reasons, the Helmholtz zeta potential is derived from that.
Fluorescence NTA provides specific information about subpopulations in the sample. A sensitive CMOS camera combined with high filtering capability and short illumination time – known as “low bleach performance” – provides ZetaView® with a high F-NTA sensitivity.
Fluorescence F-NTA AND surface charge Z-NTA in one
Both parameters, zeta potential and fluorescent light are dependent on the type of macromolecules or on the molecular composition of the particle surface.
Biologic nanoparticle with proteins of negative charge on the surface (pH=7)
Z-Two cell assemblies
The standard “NTA” cell cassette is designed for high sample throughput, whereby each user can use his own sample cell. This is also suitable for measurements in the biological safety area.
In addition to all measurement parameters, zeta potential analyzes can be carried out with the Z-NTA cassette. Both cell assemblies can be quickly and easily replaced with a slide-in / slide-out and click mechanism..
NTA cell assembly
Z – NTA cell assembly
High sample throughput
No high cost consumables
Just syringes and reference beads for scattered light and fluorescence measurements, if applicable. The life time of the quartz cell is several years. It is easy to clean with pipe cleaners.
Cleaning of Zetaview cell